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National Repository of Grey Literature

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	Regulation of development of mouse parthenogenetic embryos Jettmarová, Dominika ; Fulka, Josef (advisor) ; Kaňka, Jiří (referee) The development of mouse (Mus musculus) haploid parthenogenetic embryos does not reach the same level as normal embryos. The aim of this diploma thesis was to find out whether haploid parthenogenetic embryos of mice differ in the nucleocytoplasmic ratio. The volume of the nucleus increases with ploidity. The nucleocytoplasmic ratios of haploid embryos do not significantly change between the two-cell and four-cell stage (p = 0.052), there is a significant difference (p < 0.001) for diploid and tetraploid embryos. Non-standard nucleocytoplasmic ratio could be related to the problematic development. Understanding the regulation of preimplantational development of parthenogenetic embryos will increase the efficiency of haploid embryonic stem cell derivation. Detailed record
	Androgenesis Kočová, Helena ; Honys, David (advisor) ; Kocábek, Tomáš (referee) (anglicky) Androgenesis in the plant kingdom is an interesting phenomenon, in which a new individual is regenerated from male gametophyte. Having gametophytic, i.e. haploid number of chromosomes, these plants are potentially useful in research as well as for the generation of new genotypes. Duplication of their genetic information then results in fully homozygous plants, that can be used for breeding. At the same time, microspores represent a unique system for studying totipotency, cell proliferation, differentiation and embryogenesis. However, in many important crops as well as in some model species, such technology has not yet been efficiently managed. The aim of this thesis is to summarize the knowledge about androgenesis, from the historical context to the latest discoveries, including methods, development, complications and at the end also the possible use of obtained doubled haploid plants. Keywords: androgenesis, male gametophyte, microspore embryogenesis, pollen, totipotency, cell differentiation, stress, organogenesis, haploid Detailed record
	Regulation of development of mouse parthenogenetic embryos Jettmarová, Dominika ; Fulka, Josef (advisor) ; Kaňka, Jiří (referee) The development of mouse (Mus musculus) haploid parthenogenetic embryos does not reach the same level as normal embryos. The aim of this diploma thesis was to find out whether haploid parthenogenetic embryos of mice differ in the nucleocytoplasmic ratio. The volume of the nucleus increases with ploidity. The nucleocytoplasmic ratios of haploid embryos do not significantly change between the two-cell and four-cell stage (p = 0.052), there is a significant difference (p < 0.001) for diploid and tetraploid embryos. Non-standard nucleocytoplasmic ratio could be related to the problematic development. Understanding the regulation of preimplantational development of parthenogenetic embryos will increase the efficiency of haploid embryonic stem cell derivation. Detailed record
	Production and development of haploid mouse embryos Jettmarová, Dominika ; Fulka, Josef (advisor) ; Petelák, Aleš (referee) This bachelor thesis summarizes current knowledge of haploid embryos that are used for haploid embryonic stem cell derivation. The subject matter of this background research are production methods of haploid blastocysts of mice (Mus musculus) and the particularities of their development. Haploid blastocysts can be prepared parthenogenetically, gynogenetically or androgenetically. The development of haploid embryos is substantially different from the development of diploid embryos. The division of blastomeres is delayed by several hours and the success rate of development to blastocysts is low. The reason for the impaired development of haploid embryos is hypothesized to be improper activation and/or non-standard gene expression. The follow-up study that utilizes the Primo Vision monitoring system is described in the experimental part. Knowledge of this topic is crucial to raising the effectiveness of haploid blastocyst production and derivation of stable haploid embryonic stem cell lines for further biological and medical research. Powered by TCPDF (www.tcpdf.org) Detailed record

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